Table 5.

Stability Metrics of Proposed Novel Reference Genes and Traditional Housekeeping Genes

Compiled metrics from RT-qPCR-based reference gene stability assessments. Column 2 provides the standard deviations of aggregated D0–D10 gene expression data, with Column 3 listing the temporal statistical analysis (ANOVA significance levels during differentiation D0–D10; ns = p > 0.05, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001). Columns 4 and 5 provide the log₂-fold change between the D0–D10 aggregate data and D30–80 aggregate data (column 4) or human total heart RNA (column 5). Column 6 presents the response of gene expression to lactate treatment (Student's t-test, ns = p > 0.05, ^*p < 0.05, ^**p < 0.01; up and down refer to direction of change in expression in lactate treated vs. control samples). Column 7 lists the ANOVA significance for the “differentiation efficiency” factor of the data presented in Figure 6d. Column 8 lists the ranking of the geomean cross-parity analyses detailed in Figure 6c. Blue = column best, white = intermediate, red = column worst.

ANOVA, analysis of variance; hHrt, human heart RNA; Log2FC, log₂-fold change; RT-qPCR, real-time quantitative polymerase chain reaction; SD, standard deviation.