Figure 4.

Circ_0001944 adsorbed miR-142-5p to regulate proliferation, migration, invasion, and glycolysis of NSCLC cells. (A) Expression level of miR-142-5p in A549 and H1975 cells transfected with anti-miR-142-5p or anti-miR-NC was analyzed by qRT-PCR. (B-J) A549 and H1975 cells were transfected with sh-NC+anti-miR-NC, sh-circ_0001944+anti-miR-NC, or sh-circ_0001944+anti-miR-142-5p. (B-F) Analysis of proliferation, migration, and invasion of A549 and H1975 cells by MTT, colony formation, and transwell assays. (G and H) Evaluation of glucose uptake and lactate production in A549 and H1975 cells using an automatic biochemical analyzer. (I and J) Protein levels of HK2 and LDHA in A549 and H1975 cells were measured by Western blotting. *P < 0.05, **P < 0.01, and ***P < 0.001.