Fig. 5.

Recombinant protein variants of DHX30 induce translocation of the DHX30-WT in the cytoplasmic clusters. Immunocytochemical detection of RFP-DHX30 WT (red) and ATXN2 (magenta) after co-expression of DHX30-GFP mutants (green) in U2OS cells. Bar graph indicating the percentage of cells where RFP-DHX30 WT co-localizes with DHX30-GFP mutants within cytoplasmic clusters identified as SGs via co-staining with ATXN2 (****: significantly different form DHX30-WT: **** p < 0.0001; n > 100 from 3 independent transfections; one-way ANOVA followed by Dunnett’s multiple comparisons test). Scale bars indicate 10 μm