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. 2021 May 22;21:148. doi: 10.1186/s12906-021-03319-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of essential oils on mRNA and protein levels of IL-6 and TNFα after LPS pretreatment. BV-2 cells were pretreated with 1 μg/mL LPS for 24 h then 200-fold diluted essential oil chemotypes and standards were added to the pretreated BV-2 cells for 24 h. DMSO treatment was used as a control of the treated cells. Real-time PCR was performed with SYBR green protocol using IL-6 and TNFα specific primers. β-actin was used as housekeeping gene for the normalization and relative expression of controls was considered as 1. Proinflammatory cytokine secretions were determined using IL-6 or TNFα specific ELISA kits according to the manufacturer’s protocols. a Relative mRNA expressions of IL-6 and TNFα of the LPS pretreated cells. b IL-6 and TNFα ELISA measurements from the supernatant of the LPS pretreated BV-2 cells. The bars represent mean values and error bars represent standard deviation (SD) for three independent determinations (n = 3). Real-time PCR and ELISA measurements were carried out in triplicate in each independent experiments. Asterisks indicate p < 0.05 compared to the control. Crosses show p < 0.05 compared to the LPS treatment