Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 22;21:148. doi: 10.1186/s12906-021-03319-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — Western blot analyses of NFκB and phospho-C/EBPβ signalling pathways regulating proinflammatory cytokine production of BV-2 cells. BV-2 cells were fractionated immediately after collection and protein contents of the fractions were determined. The same amount of protein from each sample was loaded onto SDS-PAGE and transferred to nitrocellulose membranes then the membranes were probed with NFκB/p50, NFκB /p65 or P-C/EBPβ polyclonal rabbit antibodies according to the manufacturer’s protocol. β-actin was used as housekeeping control. a Protein levels of p50, p65 and P-C/EBPβ after LPS pretreatment. b Protein levels of p50, p65 and P-C/EBPβ after essential oil pretreatment. c Protein levels of p50, p65 and P-C/EBPβ after LPS and essential oil co-treatment. d-f Optical densities of the Western blot analyses of p50, p65 and P-C/EBPβ after the different treatments. The Western blots were analysed using ImageJ software, the optical density of the examined proteins was expressed as percentage of target protein/β-actin abundance. The bars represent mean values and error bars represent standard deviation (SD) for three independent experiments (n = 3). Asterisks indicate p < 0.05 compared to the control. Crosses show p < 0.05 compared to the LPS treatment. The protein samples from the same experiment were separated on different gels and only the target protein is visible on the blot used for the figure. Full-length blots are presented in Supplementary Fig. 1