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. 2021 May 22;18:118. doi: 10.1186/s12974-021-02171-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — No direct effect was detected in the activation of neutrophil and monocyte after stimulation by the BMVEC supernatant. a, b In vitro experiment design: the collected BMVEC supernatant was added into the HL-60 cell line or THP-1 culture media and cultured for 72 h. b ROS production in HL-60 was tested. *** vs. LV Vehicle + OGD indicate p < 0.001. c NET formation in HL-60 was detected by ELISA. **** vs. LV Vehicle + OGD indicate p < 0.0001. d ROS production in THP-1 was tested. ** and *** vs. LV Vehicle + OGD indicate p < 0.01 and 0.001, respectively. e sCD14 secretion in THP-1 supernatant was detected by ELISA. * vs. LV Vehicle + OGD indicate p < 0.05. f sCD163 secretion in THP-1 supernatant was detected by ELISA. *** vs. LV Vehicle + OGD indicate p < 0.001