. 2021 May 22;20:38. doi: 10.1186/s12941-021-00443-w

Table 2.

Performance characteristics of six nucleic acid amplification kits for the detection of SARS-CoV-2 using clinical diagnostic reports as the reference standard

	No. of RT-qPCR result	No. of clinical diagnostic report		Sensitivity	Specificity	PPV	NPV	Diagnostic accuracy	Kappa
	No. of RT-qPCR result	Positive	Negative	(95% CI)	(95% CI)	(95% CI)	(95% CI)	(95% CI)	Kappa
A	Positive	26	0	100 (87.1–100)	100 (87.1–100)	100 (87.1–100)	100 (87.1–100)	100	1
A	Negative	0	26	100 (87.1–100)	100 (87.1–100)	100 (87.1–100)	100 (87.1–100)	(93.1–100)	(p < 0.001)
B	Positive	23	0	100 (85.7–100)	89.7 (73.6–96.4)	88.5 (71.0–96.0)	100 (87.1–100)	94.2	0.885
B	Negative	3	26	100 (85.7–100)	89.7 (73.6–96.4)	88.5 (71.0–96.0)	100 (87.1–100)	(84.4–98.0)	(p < 0.001)
C	Positive	25	0	100 (86.7–100)	96.3 (81.7–99.3)	96.2 (81.1–99.3)	100 (87.1–100)	98.1	0.962
C	Negative	1	26	100 (86.7–100)	96.3 (81.7–99.3)	96.2 (81.1–99.3)	100 (87.1–100)	(89.9–99.7)	(p < 0.001)
D	Positive	18	0	100 (82.4–100)	76.5 (60.0–87.6)	69.2 (50.0–83.5)	100 (87.1–100)	84.6	0.692
D	Negative	8	26	100 (82.4–100)	76.5 (60.0–87.6)	69.2 (50.0–83.5)	100 (87.1–100)	(72.5–92.0)	(p < 0.001)
E	Positive	20	0	100 (83.9–100)	81.3 (64.7–91.1)	76.9 (57.6–89.0)	100 (87.1–100)	88.5	0.769
E	Negative	6	26	100 (83.9–100)	81.3 (64.7–91.1)	76.9 (57.6–89.0)	100 (87.1–100)	(77.0–94.6)	(p < 0.001)
F	Positive	24	0	100 (86.2–100)	92.9 (77.4–98.0)	92.3 (75.9–97.9)	100 (87.1–100)	96.15	0.923
F	Negative	2	26	100 (86.2–100)	92.9 (77.4–98.0)	92.3 (75.9–97.9)	100 (87.1–100)	(87.0–98.9)	(p < 0.001)