Figure 5.

Effects of T supplementation on body weight and body composition of HFD-fed chemically castrated male N-ERαKO mice. Male N-ERαKO mice were injected with degarelix and supplemented with placebo (DGX, n = 6) or T (DGX + T, n = 8) at 16 weeks of age and subsequently fed with HFD for 20 weeks. A: Body weight and body composition of 16 to 36-week-old mice (B–C). Fat (D) and lean (E) body mass were normalized to body weight. Data were analyzed with two-way ANOVA with Bonferroni post-hoc test. *P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001. Data are represented as mean ± SEM. Abbreviations: ANOVA, analysis of variance; BW, body weight; DGX, degarelix; DHT, dihydrotestosterone; HFD, high fat diet; N-ErαKO, extrahypothalamice neuron-specific estrogen receptor α knockout; SEM, standard of error; T, testosterone.