Figure 7.

Effects of T supplementation on physical activity and energy balance of HFD-fed chemically castrated male N-ERαKO mice. Food intake (A), ambulatory movement (B), respiratory exchange ratio (C), oxygen consumption (D), and heat production (E) as measured by indirect calorimetry in 36-week-old N-ERαKO male mice injected with degarelix and supplemented with placebo (DGX, n = 6) or T (DGX + T, n = 8) at 16 weeks of age and subsequently fed with HFD for 20 weeks. D, E (right panels): Oxygen consumption and heat production were normalized to lean body mass. Data were analyzed with two-way ANOVA with Bonferroni post hoc test. ^**P < 0.01, ^***P < 0.001. Data are represented as mean ± SEM. Abbreviations: ANOVA, analysis of variance; DGX, degarelix; HFD, high fat diet; N-ERαKO = extrahypothalamice neuron specific estrogen receptor α knockout; RER, respiratory exchange ratio; SEM, standard error of the mean; T, testosterone; VO2,  oxygen consumption.