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. 2021 May 22;26(5):056004. doi: 10.1117/1.JBO.26.5.056004

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© 2021 The Authors

Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Fig. 1 — Schematic overview of the experimental setup which allows for simultaneous blood flow measurements using LSCI and total RBF. The LSCI setup consists of a grayscale camera with optics, a red 638-nm laser with a diffuser, and a computer to run the LSCI algorithm. The laser light homogenously illuminates the kidney, which allows for full-field, real-time perfusion imaging of the cortical blood flow. The kidney is connected to a computer that measures and regulates the total RBF, pressure, and temperature. The autologous blood is oxygenated using carbogen and warmed using a warm water bath. The syringe pump is used to add compounds to keep the blood composition stable. The urine is collected to monitor urine production.