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H&E staining (A), 100×, and IHC, (B) for CK20 (+), (C) for Ki-67 (−), and (D) for IHC with D5F3 anti-ALK Ventana antibody showing strong staining and verifying ALK overexpression as a result of EML4-ALK fusion. ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry.
