Figure 7.

Effects of GPS on the PI3K/Akt/Rho-kinase pathway. (a) Protein expression levels of p-Akt, Akt, p-MYPT1, MYPT1, p-MLC, and MLC in aortic rings without endothelium were analyzed using western blotting. Data are presented as SEM (n = 3). ^∗p < 0.05 compared to the control group; ^#p < 0.05 compared to the PE group. (b) Endothelium-denuded aortic rings were preincubated with verapamil, LY294002, and Y27632, and then, the rings were treated with GPS (20, 80, and 160 μΜ) after precontracted with PE. Data are presented as mean ± SEM (n = 6). ^∗p < 0.05 compared to the control group.