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. 2021 May 14;2021:6652775. doi: 10.1155/2021/6652775

Table 1.

List of forward (F) and reverse (R) primer sequences and TaqMan probes (Pr) used for real-time RT-PCR.

Gene (the encoded protein) GenBank accession number Primer sequence
Gapdh (housekeeping gene) NM_001289726.1 F: 5′-AACTTTGGCATTGTGGAAGGGCTC-3′
R: 5′-ACCAGTGGATGCAGGGATGATGTT-3′
Pr: R6G-5′-ATGACCACAGTCCATGCCATCACTGCCA-3′-Q
Nfe2l2 (transcription factor Nrf2) NM_010902.4 F: 5′-TGATGGACTTGGAGTTGC-3′
R: 5′-TCAAACACTTCTCGACTTACT-3′
Pr: Fam-5′-TACAGTCCCAGCAGGACATGGATT-3′-Q
Nqo1 (NAD(P)H : quinone oxidoreductase 1) NM_008706.5 F: 5′-CGAATCTGACCTCTATGCTATG-3′
R: 5′-TAGAGATGACTCGGAAGGATAC-3′
Pr: Fam-5′-TGAGCTGAAGGACTCGAAGAACT-3′-Q
Hmox1 (heme oxygenase 1) NM_010442.2 F: 5′-AGACCGCCTTCCTGCTCAACATT-3′
R: 5′−TGACGAAGTGACGCCATCTGTGA-3′
Pr: Fam-5′-TGAGGAGCTGCAGGTGATGCTGACAGAGGA-3′-Q
Gstp1 (glutathione S-transferase P1) NM_013541.1 F: 5′-GATGGAGACCTCACCCTTTA-3′
R: 5′-CCCATCATTCACCATATCCATC-3′
Pr: Fam-5′-CTAATGCCATCTTGAGACACCTTGG-3′-Q
Rela (nuclear factor κB p65 subunit) NM_009045 F: 5′-CAGATACCACCAAGACACAC-3′
R: 5′-ACAAGTTCATGTGGATGAGG-3′
Pr: Fam-5′-AATGGCTACACAGGACCAGGAACA-3′-Q
Nfκb2 (nuclear factor κB p100 subunit) NM_019408.3 F: 5′-CTTACTCGCCTCCTTCTAAA-3′
R: 5′-TTTCTTTGGGTATCCCTCTC-3′
Pr: Fam-5′-ATCCATGCAGAGAATGAGGAGCCT-3′-Q
Jun (jun protooncogene, AP-1) NM_010591.2 F: 5′-ACGGAGAAGAAGCTCACAA-3′
R: 5′-GTCCTCTGGGTCAGGAAAG-3′
Pr: Fam-5′-AGGAAATAGGCGAGCGGCTACC-3′-Q
Becn1 (beclin 1, autophagy related) NM_019584.3 F: 5′-AGAGGCTAACTCAGGAGA-3′
R: 5′-CCGATCAGAGTGAAGCTATTA-3′
Pr: Fam-5′-AAACTCGCCAGGATGGTGTCTCTC-3′-Q
Map1lc3b (microtubule-associated protein 1 light chain 3 beta, LC3B) NM_026160.4 F: 5′-GAAGTGTACGAGAGTGAGAGAGA-3′
R: 5′-CAACCATTGGCTTTGTTGGAG-3′
Pr: Fam-5′-CGCAGGAGACATTCGGGACAGCAA-3′-Q
Tfeb (transcription factor EB) NM_011549.3 F: 5′-CCCTGTCCACTTCCAGTC-3′
R: 5′-GATACTCCCGAACCTTCTGATG-3′
Pr: Fam-5′-TCCTACCACCTGCAACAGTCCCA-3′-Q
Tfe3 (transcription factor E3) NM_172472.3 F: 5′-CACACTGAGTCGTCCACCT-3′
R: 5′-GACAAGTACTGTTTGACCTGCTG-3′
Pr: Fam-5′-AACCCTACACGCTACCACCTGCA-3′-Q