Table 1.
Gene (the encoded protein) | GenBank accession number | Primer sequence |
---|---|---|
Gapdh (housekeeping gene) | NM_001289726.1 | F: 5′-AACTTTGGCATTGTGGAAGGGCTC-3′ R: 5′-ACCAGTGGATGCAGGGATGATGTT-3′ Pr: R6G-5′-ATGACCACAGTCCATGCCATCACTGCCA-3′-Q |
Nfe2l2 (transcription factor Nrf2) | NM_010902.4 | F: 5′-TGATGGACTTGGAGTTGC-3′ R: 5′-TCAAACACTTCTCGACTTACT-3′ Pr: Fam-5′-TACAGTCCCAGCAGGACATGGATT-3′-Q |
Nqo1 (NAD(P)H : quinone oxidoreductase 1) | NM_008706.5 | F: 5′-CGAATCTGACCTCTATGCTATG-3′ R: 5′-TAGAGATGACTCGGAAGGATAC-3′ Pr: Fam-5′-TGAGCTGAAGGACTCGAAGAACT-3′-Q |
Hmox1 (heme oxygenase 1) | NM_010442.2 | F: 5′-AGACCGCCTTCCTGCTCAACATT-3′ R: 5′−TGACGAAGTGACGCCATCTGTGA-3′ Pr: Fam-5′-TGAGGAGCTGCAGGTGATGCTGACAGAGGA-3′-Q |
Gstp1 (glutathione S-transferase P1) | NM_013541.1 | F: 5′-GATGGAGACCTCACCCTTTA-3′ R: 5′-CCCATCATTCACCATATCCATC-3′ Pr: Fam-5′-CTAATGCCATCTTGAGACACCTTGG-3′-Q |
Rela (nuclear factor κB p65 subunit) | NM_009045 | F: 5′-CAGATACCACCAAGACACAC-3′ R: 5′-ACAAGTTCATGTGGATGAGG-3′ Pr: Fam-5′-AATGGCTACACAGGACCAGGAACA-3′-Q |
Nfκb2 (nuclear factor κB p100 subunit) | NM_019408.3 | F: 5′-CTTACTCGCCTCCTTCTAAA-3′ R: 5′-TTTCTTTGGGTATCCCTCTC-3′ Pr: Fam-5′-ATCCATGCAGAGAATGAGGAGCCT-3′-Q |
Jun (jun protooncogene, AP-1) | NM_010591.2 | F: 5′-ACGGAGAAGAAGCTCACAA-3′ R: 5′-GTCCTCTGGGTCAGGAAAG-3′ Pr: Fam-5′-AGGAAATAGGCGAGCGGCTACC-3′-Q |
Becn1 (beclin 1, autophagy related) | NM_019584.3 | F: 5′-AGAGGCTAACTCAGGAGA-3′ R: 5′-CCGATCAGAGTGAAGCTATTA-3′ Pr: Fam-5′-AAACTCGCCAGGATGGTGTCTCTC-3′-Q |
Map1lc3b (microtubule-associated protein 1 light chain 3 beta, LC3B) | NM_026160.4 | F: 5′-GAAGTGTACGAGAGTGAGAGAGA-3′ R: 5′-CAACCATTGGCTTTGTTGGAG-3′ Pr: Fam-5′-CGCAGGAGACATTCGGGACAGCAA-3′-Q |
Tfeb (transcription factor EB) | NM_011549.3 | F: 5′-CCCTGTCCACTTCCAGTC-3′ R: 5′-GATACTCCCGAACCTTCTGATG-3′ Pr: Fam-5′-TCCTACCACCTGCAACAGTCCCA-3′-Q |
Tfe3 (transcription factor E3) | NM_172472.3 | F: 5′-CACACTGAGTCGTCCACCT-3′ R: 5′-GACAAGTACTGTTTGACCTGCTG-3′ Pr: Fam-5′-AACCCTACACGCTACCACCTGCA-3′-Q |