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Photomicrographs of slides stained with periodic acid Schiff (PAS). Oxidized hydroxyl and amine/alkylamine chemical groups appear as a magenta-colored complex. The detection of polysaccharides, glycoproteins, and glycolipids suggests new bone formation. (A) bone defects filled with hydroxyapatite; (B) bone defects filled with nano-hydroxyapatite/ß tricalcium phosphate; (C) defect without biomaterial (control). Scale bar 100 µm, X 400 magnification.
