Figure 1.

Semi-quantitative counting scheme to determine activation of NF-κB in epithelial cells and recruitment of CD45-positive cells into the lamina propria. Example of images used to quantify epithelial cells with labeled nuclear NF-kB (arrowheads) or no nuclear NF-κB labeling (asterisks) in jejunum of uninfected (A) and C. suis infected (B) piglets. Example of images used to quantify CD45-positive (arrowheads) and CD45-negative (asterisks) labeling in cells of the lamina propria in jejunum of uninfected (C) and C. suis infected (D) piglets (solid black line indicates boundary of epithelium and lamina propria, dashed black line delimits epithelium). Scale bar 40 µm.