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. 2021 May 18;14:2031–2043. doi: 10.2147/JIR.S302832

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© 2021 Guo et al.

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Identification of LIF as a direct target of miR-29c-3p. (A) Schematic description of a putative binding site of miR-29c-3p in the 3ʹ-UTR of human LIF. (B) Luciferase reporter activity in Caco-2 cells. (C) QRT-PCR analysis of miR-29c-3p expression in Caco-2 cells pre-transfected with miR-29c-3p mimics, miR-29c-3p inhibitor or corresponding scramble controls. (D) qRT-PCR analysis of LIF mRNA levels in pre-transfected Caco-2 cells. (E) Representative Western blots and densitometric quantitative analysis of LIF protein levels in pre-transfected Caco-2 cells. ** and *** indicates P<0.01 and 0.001, respectively.