Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-ATM | Cell Signaling | Cat#2873 |
| anti-β-Actin | Cell Signaling | Cat#3700 |
| anti-β-catenin | Upstate | Cat#06–734 |
| anti-cMYC | Santa Cruz Biotech | Cat#sc-40 |
| anti-FLAG | Sigma-Aldrich | Cat#F3165 |
| anti-GAPDH | Abcam | Cat#ab9485 |
| anti-HDAC2 | Santa Cruz Biotech | Cat#sc-7899 |
| anti-HIPK2 | Abcam | Cat#ab75937 |
| Anti-LEF1 | Bethyl Laboratories | Cat#A303–486A |
| anti-phospho-Y361-HIPK2 | Thermo Scientific | Cat#PA5–13045 |
| anti-phospho-S209-TCF3 | Sergei Sokol (Hikasa and Sokol, 2011) | N/A |
| anti-phospho-S46-p53 | Abcam | Cat#ab76242 |
| anti-phospho-S15-p53 | Cell Signaling | Cat#9284 |
| anti-TCF4 | Abcam | Cat#ab217668 |
| anti-TCF4 (for ChIP qPCR) | Santa Cruz Biotechnologies | Cat# sc-8631 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| miRVana isolation kit | Thermo Fisher | Cat# AM1560 |
| SYBR Green Supermix | BioRad | Cat#1708882 |
| iTaq SYBR Green Supermix | Bio-Rad | Cat#1725120 |
| QuantiTect reverse transcription kit | Qiagen | Cat# 205311 |
| Recombinant human Wnt3a | R&D Systems | Cat#5036-WN |
| Primary screen library | Sanford-Burnham-Prebys Medical Discovery Institute Chemical Genomics core facility | https://www.sbpdiscovery.org/medical-discovery/drug-discovery/prebys-center-for-drug-discovery/overview) |
| Deposited Data | ||
| Not applicable | ||
| Experimental Models: Cell Lines | ||
| pBARLRen cell line (derived from RKO cells) | Moon lab, University of Washington (Biechele et al., 2009) | N/A |
| NF-kB reporter (HEK293-NF-kB-Luc) stably expressing NOD1 | John C. Reed, | https://pubchem.ncbi.nlm.nih.gov/bioassay/1290 |
| HEK293T | ATCC | Cat#CRL-3216 |
| SW480 | ATCC | Cat#CCL-228 |
| Oligonucleotides | ||
| Primers for ChIP qPCR, see Table S3 | This paper | N/A |
| Primers for qRT-PCR, see Table S4 | This paper | N/A |
| Taqman primers for Taqman qRT-PCR, see Table 2 | Thermo Fisher | See Table 2 |
| Recombinant DNA | ||
| pcDNA-Wnt3a | (Najdi et al., 2012) | Addgene Cat#35908 |
| MYC-tagged TCF4 | (Korinek et al., 1997) | Addgene Cat#16512 |
| Constitutively active mutant b-catenin | (Taelman et al., 2010) | Addgene Cat#29684 |
| wildtype b-catenin | (Lee et al., 2001) | Addgene Cat#13434 |
| pcDNA3β-FLAG-CBP-HA | (Zhao et al., 2005) | Addgene Cat#32908 |
| pCMVβ-p300-MYC | (Addgene depositor Tso-Pang Yao) | Addgene Cat#30489 |
| pCTX-myc-HIPK2 WT | (Hikasa et al., 2010) | Addgene Cat#32996 |
| TCF1-FLAG | Sergei Sokol (Hikasa and Sokol, 2011) | N/A |
| TCF3-FLAG | Sergei Sokol (Hikasa and Sokol, 2011) | N/A |
| TCF4-FLAG | Sergei Sokol (Hikasa and Sokol, 2011) | N/A |
| LEF1-FLAG | Sergei Sokol (Hikasa and Sokol, 2011) | N/A |
| Software and Algorithms | ||
| ChIP-IT qPCR Analysis Kit | Active Motif | https://www.activemotif.com/catalog/872/chip-it-qpcr-analysis-kit |
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ChemDraw Ultra version 11 | PerkinElmer | https://www.perkinelmer.com/ |
| Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
| Excel | Microsoft | https://www.microsoft.com/en-us/microsoft-365/excel |
| Biorender | Biorender | https://biorender.com/ |