Figure 6.

¹⁵N NMR spectra of uniformly ¹⁵N labeled membrane-bound coat protein of Pf1 bacteriophage in magnetically aligned q=5.0 DMPC/Triton X-100 bicelles. The bilayer normal is perpendicular to the direction of the magnetic field. The spectra were obtained with double-ramped adiabatic cross-polarization. A. 200 μL sample in a 5 mm OD 3D printed chamber. C. Expanded section of A. between the two arrowheads. B. Same as C., except that 150 μL of the same sample is contained in a 5 mm OD glass sample tube inserted in the solenoid coil of the dual-coil probe. The expanded spectral regions shown in B. and C are from spectra resulting the co-addition of 4,000 free induction decays and processed using identical squared sine bell functions. The protein concentration in both samples is 1.43 mg/mL.