Skip to main content
. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Neuron. 2021 Apr 7;109(10):1636–1656.e8. doi: 10.1016/j.neuron.2021.03.016

Figure 4. Ophn1 Deletion Selectively in PV INs in PL-mPFC is Sufficient to Induce LH.

Figure 4.

(A) AAV vector expressing flippase-dependent Cre-GFP (AAV-fDIO-Cre-GFP) for gene deletion with spatial and cell-type specificity.

(B) Schematic for depleting Ophn1 in PV INs in the PL-mPFC of Ophn1flox/Y;PV-Flp mice (PL, prelimbic; IL, infralimbic; DP, dorsal peduncular; ACd, dorsal anterior cingulate).

(C) Representative images of neurons infected with control AAV-fDIO-mCherry or AAV-fDIO-Cre-GFP in the PL-mPFC. Scale bar, 200 μm.

(D) Representative images showing selective depletion of OPHN1 in PL-mPFC PV INs of Ophn1flox/Y;PV-Flp mice injected with AAV-fDIO-Cre-GFP but not AAV-fDIO-mCherry. Scale bar, 10 μm.

(E) LH test of Ophn1flox/Y;PV-Flp mice in which the PL-mPFC was injected with AAV-fDIO-mCherry or AAV-fDIO-Cre-GFP. n = 19–25 mice per group; Mann-Whitney test. Data are presented with the median and interquartile ranges shown in red. Closed and open circles represent LH and resilient mice, respectively. Percentage of LH+ mice is indicated at the bottom of each group. **p < 0.01.

See also Figure S4.