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. 2021 May 22;12(6):527. doi: 10.1038/s41419-021-03811-8

Fig. 6. Thoracic IR significantly elevated the expression of fibrotic genes and SASP genes in senescent macrophages of right lung tissue.

Fig. 6

C57BL/6J mice were exposed to a single dose of 17 Gy IR on the right side of the thorax. Samples of right lung tissue were collected at intervals after IR and in control. The macrophages were sorted by FCM. The expression of A fibrotic genes (Tgf-β1 and Arg-1), B SASP proinflammatory factors genes (Il-1α, Il-6, and Tnf-α), C SASP chemokines genes (Ccl2, Cxcl10, and Ccl17), D SASP Mmps genes (Mmp2, Mmp9, and Mmp12) were quantified by qRT–PCR and were expressed relative to Gapdh levels in isolated macrophages. Data are expressed as mean ± SEM of six independent experiments, ***P < 0.001, **P < 0.01, and *P < 0.05 compared with the corresponding control.