Skip to main content
. 2021 May 22;12(6):528. doi: 10.1038/s41419-021-03820-7

Fig. 3. PPARG2 suppresses cell proliferation and induces cell cycle arrest of PCA cells in vitro.

Fig. 3

A CCK-8 assay of the proliferation rates of recombinant PCA cell lines transfected with EV- and PPARG2-overexpression lentivirus vectors, respectively. *P < 0.01. B, C EDU assays were performed in EV and PPARG2-transfected PCA cells. *P < 0.05 vs. EV group. D, E Cell cycle profiles were examined by flow cytometry with propidium iodide (PI) staining; cell numbers were counted according to DNA content of G1, S, and G2 phases. *P < 0.05 vs. EV group. FH Western blot analysis of cyclinD1, cyclinB1, p21Cip1 and p27Kip1, Bcl-2, p-AKT, and AKT protein expressions in indicated cells. *P < 0.05 vs. EV group.