Skip to main content
. 2021 May 22;12(6):528. doi: 10.1038/s41419-021-03820-7

Fig. 7. Experimental verification of the relationship between DNMT3A/3B and miR-200b-3p.

Fig. 7

A miR-200b-3p expression extracted from miRNA-seq results (n = 3, P = 0.008). B Relative miR-200b-3p expression in vitro when overexpression of PPARG2. *P < 0.05 vs. EV group. C Putative miR-200b-3p-binding 3′-UTR sequence of DNMT3A/3B mRNA. Mutation was generated on the DNMT3A/3B mRNA 3′-UTR sequence in the complementary site for the seed region of miR-200b-3p. The wild-type or mutant miR-200b-3p-binding DNMT3A/3B mRNA 3′-UTR sequence was cloned into pmiR luciferase reporter. D The wild-type (DNMT3A/3′-UTR-WT) and mutant (DNMT3A/3′-UTR-Mut) pmiR luciferase reporter were co-transfected into PC3 cells with miR-CON and miR-200b-3p. *P < 0.05 vs. miR-CON group. E The wild-type (DNMT3B/3′-UTR-WT) and mutant (DNMT3B/3′-UTR-Mut) pmiR luciferase reporter were co-transfected into PC3 cells with miR-CON and miR-200b-3p. *P < 0.05 vs. miR-CON group. F Relative miR-200b-3p expression in vitro transfected with miR-200b-3p mimic or miR-200b-3p inhibitor by RT-qPCR. U6 was used as an internal control. *P < 0.05 vs. miR-CON group, #P < 0.05 vs. scramble group. G, H Analysis of DNMT3A protein expression by western blotting when transfected with miR-CON, miR-200b-3p mimic, scramble, or miR-200b-3p inhibitor into PC3 cells. β-Actin was used as an internal control. *P < 0.05 vs. miR-CON group, #P < 0.05 vs. scramble group. I, J Analysis of DNMT3B protein expression by western blotting when transfected with miR-CON, miR-200b-3p mimic, scramble, or miR-200b-3p inhibitor into PC3 cells. β-Actin was used as an internal control. *P < 0.05 vs. miR-CON group, #P < 0.05 vs. scramble group.