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. 2021 May 22;21:273. doi: 10.1186/s12935-021-01984-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Circ_0006168 regulated JMJD1C expression by acting as a sponge of miR-194-5p. A Predicted binding sites between miR-194-5p and circ_0006168 were shown. B The expression of miR-194-5p was analyzed by qRT-PCR in Eca109/Taxol and KYSE150/Taxol transfected with si-control and si-circ_0006168. C The level of miR-194-5p was examined by qRT-PCR in Eca109/Taxol and KYSE150/Taxol cells transfected with miR-NC or miR-194-5p. D Dual-luciferase reporter assay was performed to determine the luciferase activity in Eca109/Taxol and KYSE150/Taxol cells co-transfected with miR-NC or miR-194-5p and circ_0006168-wt or circ_0006168-mut. E Circ_0006168 and miR-194-5p enrichment in Eca109/Taxol and KYSE150/Taxol were analyzed by RIP assay. F Circ_0006168 enrichment in Eca109/Taxol and KYSE150/Taxol transfected with NC-input, bio-NC, bio-miR-194-5p, or miR-194-5p-input was detected using RNA pull-down assay. G The level of miR-194-5p was detected by qRT-PCR in ESCC tissues and adjacent normal tissues. H The expression of miR-194-5p was examined by qRT-PCR in HET-1A cells, parental cells (Eca109 and KYSE150), and Taxol-resistant cells (Eca109/Taxol and KYSE150/Taxol). I The putative binding sites between miR-194-5p and JMJD1C were predicted by TargetScan. J The luciferase activity was determined in Eca109/Taxol and KYSE150/Taxol co-transfected with miR-NC or miR-194-5p and JMJD1C-wt or JMJD1C-mut. K JMJD1C and miR-194-5p enrichment in Eca109/Taxol and KYSE150/Taxol were measured by RIP assay. L Circ_0006168 enrichment was determined by RNA pull-down assay in Eca109/Taxol and KYSE150/Taxol cells transfected with NC-input, bio-NC, bio-miR-194-5p, or miR-194-5p-input. M The expression of miR-194-5p was examined by qRT-PCR Eca109/Taxol and KYSE150/Taxol cells transfected with inhibitor-NC or inhibitor-miR-194-5p. N The protein expression of JMJD1C was tested by western blot analysis in Eca109/Taxol and KYSE150/Taxol transfected with miR-NC, miR-194-5p, inhibitor-NC, or inhibitor-miR-194-5p. O Western blot assay was carried out to analyze the protein expression of JMJD1C in Eca109/Taxol and KYSE150/Taxol transfected with si-control, si-circ_0006168, si-circ_0006168 + inhibitor-NC, si-circ_0006168 + inhibitor-miR-194-5p. *P < 0.05, **P < 0.001, ***P < 0.0001