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. 2021 Feb 10;6(1):e01090-20. doi: 10.1128/mSphere.01090-20

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Copyright © 2021 Vélez-Ramírez et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — APEX2 directs organelle-specific biotinylation in T. brucei. (A) Western blot of whole-cell lysate (W), NP-40-extracted supernatant (S), and pellet (P) samples from 29-13 and DRC1-APEX2-expressing cells. Samples were probed with anti-HA antibody. (B) Samples in panel A were stained with SYPRO Ruby to assess loading. (C) 29-23 and DRC1-APEX2 cells were examined by immunofluorescence with anti-PFR antibody (Alexa 488, green), streptavidin (Alexa 594, red) and DAPI (blue). Boxes show zoomed-in versions of the cells. Brackets point out that streptavidin (Alexa 594, red) extends up to the kinetoplast. Scale bar, 5 μm.