TABLE 2.
AC1s proximity proteomea
| TriTryp GeneID | TriTryp annotation | Localizationb | Reference and/or source for location data | AC1s/FS179s avg ratioc |
|---|---|---|---|---|
| Tb927.2.5860 | Hypothetical | Tip | TrypTag (48) | 15.88 |
| Tb927.2.5870 | Hypothetical | Tip | TrypTag (48, and this work) | 10.71 |
| Tb927.4.1500 | RNA editing associated helicase 2 | Not flagellar | TrypTag (48) | 7.45 |
| Tb927.4.4400 | Hypothetical | Flagellum, distal points plus endocytic | TrypTag (48) | 5.18 |
| Tb927.2.5760 | Flagellar member 8 | Tip | 37 | 5.03 |
| Tb927.11.14410 | Ankyrin repeats | Not flagellar | TrypTag (48) | 3.89 |
| Tb927.1.2120 | Calpain-like protein CALP1.3 | Tip | 62 | 3.60 |
| Tb927.11.17040 | AC1d | Tip | 32 | 3.37 |
| Tb927.7.4060 | Cysteine peptidase, clan CA, family C2 | Flagellum, tip enriched plus cytoplasm | TrypTag (48, 62) | 3.02 |
| Tb927.9.7540 | Cysteine peptidase, clan CA, family C2, putative | Flagellum, tip enriched | TrypTag (48, and this work) | 2.98 |
| Tb927.7.4070 | Cysteine peptidase, clan CA, family C2 | Tip plus cytoplasm | 62 | 2.86 |
| Tb927.7.5340 | cAMP response protein 3 | Flagellum, tip enriched plus cytoplasm | TrypTag (48, and this work) | 2.41 |
| Tb927.10.15700 | Hypothetical | Tip | 2.11 | |
| Tb927.4.4220 | Small GTP-binding rab protein | Flagellar pocket | TrypTag (48) | 2.11 |
| Tb927.10.9380 | SEP domain/UBX domain containing protein | New flagellum-distal points | TrypTag (48) | 1.86 |
| Tb927.5.2090 | Kinesin (KIN2A) | Flagellum, tip (weak) plus cytoplasm | TrypTag (48) | 1.74 |
| Tb927.6.4710 | Calmodulin | Flagellum | TrypTag (48) | 1.68 |
| Tb927.3.4640 | VIT family | Not flagellar | TrypTag (48) | 1.50 |
| Tb927.7.7260 | Kinesin (KIF9B) | Flagellum puncta, basal and probasal body | 92 | 1.32 |
| Tb927.9.9690 | Hypothetical | Flagellum, tip enriched | TrypTag (48) | 1.22 |
| Tb927.7.3090 | Galactose oxidase, central domain containing protein, putative | Not flagellar | TrypTag (48) | 1.17 |
| Tb927.5.2410 | (Tip) kinesin (KIN-E) | Flagellum, tip enriched | TrypTag (48, 61) | 1.16 |
a
Proteins in boldface font have a known role in cell signaling.
b
Tip, the prominent or sole location is flagellum tip; flagellum, tip enriched, in the flagellum and enriched at tip; tip enriched, enriched at the flagellum tip, but also present outside the flagellum.
c
Normalized spectral count average of all experiments and replicates of AC1s over normalized spectral count average of all experiments and replicates of FS179s. Only proteins with AC1/FS179 ratio of >1 are included in the AC1s proteome.
d
AC1 represents a total of 27 ACs identified in the AC1s proximity proteome (see Table S4 in the supplemental material).