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. 2021 May 20;9(5):e001912. doi: 10.1136/jitc-2020-001912

Figure 2.

Figure 2

Vaccination activates mature and terminally differentiated NK cells. KIR-Tg mice were injected with two doses of the indicated DNA construct 1 week apart and then assessed for expression of KLRG1 on CD11b, CD27 NK cell subsets from the spleens and livers 1 week after the final injection. (A–D) KLRG1 on splenic CD11b+CD27+ (A) and CD11b+CD27− (B) NK cells and on hepatic CD11b+CD27+ (C) and CD11b+CD27− (D) NK cells following vaccination. N=7–14 mice per group. (E) CD69 expression on KIR2DS2-positive CD11b CD27 NK cell subsets (n=3 per group). (F, G) Comparison of KLRG1 expression on KIR2DS2+ and KIR2DS2− splenic NK cells in the CD11b+CD27+ (F) and CD11b+CD27− (G) subpopulations. N=7–14 mice per group. (H) KIR-Tg mice crossed with C57BL/6 mice were injected with two doses of the indicated DNA construct 1 week apart and then assessed for expression of KLRG1 on total NK cells, and CD11b, CD27, NK cell 2DS2+ and 2DS2− subsets from their spleens. N=4–6 mice per group. Comparisons between two groups were made by paired t-test (two groups) (E–G) and two‐way analysis of variance with Dunnett’s test for multiple comparisons to compare individual groups (A–D and H) (*p<0.05, **p<0.01, ***p<0.005, ****p<0.001). KIR, killer cell immunoglobulin-like receptor; NK, natural killer.