Figure 4.

Proportion of main immune cells in peripheral organs of CJ mice, Bmal1^-/-mice, and Per1^-/-Per2^-/-mice. (A–D) Proportion of B220⁺ cells, CD3⁺ cells, CD11c⁺ cells, CD49b⁺ cells, and CD11b⁺ cells in peripheral organs of CJ mice, Bmal1^-/- mice, and Per1^-/-Per2^-/- mice. (E–H) Proportion of subpopulations of B cells in peripheral organs of CJ mice, Bmal1^-/- mice, and Per1^-/-Per2^-/- mice. (I) Fluorescence-activated cell sorter (FACS) graphs showing B220⁺CD1d⁺CD5⁺ cells, MFI of IL10 and IL33 on B220⁺CD1d⁺CD5⁺ cells in IELs from DSS-treated or untreated Bmal1^-/- and WT mice. (J) Proportion of CD1d⁺CD5⁺cells of B220⁺ cells in IELs. (K) MFI of IL33 and (L) IL10 on B220⁺CD1d⁺CD5⁺ in IELs. (M) Rhythmic oscillation of proportion of B220⁺CD1d⁺CD5⁺ cells in the IELs and (N) hepatic lymphocytes from DSS-treated Bmal1^-/- and WT mice within 24 hours. The P value is estimated by JTK cycle analysis. FSC, forward scatter; NC, non-specific control; PE, Phycoerythrin; SSC, side scatter.