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. 2021 May 24;7:40. doi: 10.1186/s40729-021-00327-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Using a digital drawing and crop tool the regions of interest (ROIs) were segmented relative to the adjacent structures (a). The yellow line designates the lateral area inward (l₁) or outward (l₂) of middle sagittal seam of the newly formed bone and the green line designates the area of central bone formation (c), while the blue circular line specifies the area of the remained biomaterial (BmA). Τhe total number of osteocytes (Ost-arrows) in the newly formed bone surface was identified and used to estimate the osteocyte cell density (CD; Ost/mm²) in each ROI of both experimental and control groups (b) [hematoxylin and eosin stain, original magnifications for A × 20. Inset figure b was prepared using ObjectiveViewer (https://www.objectivepathology.com/objectiveview)]