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. 2021 Jan 29;384(2):287–299. doi: 10.1007/s00441-020-03328-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–h Photomicrographs of whole-mount preparations of myenteric plexus (MP) of rat ileum (a–d) and colon (e–h) showing AT8 immunolabeling in control normothermic (CTRL) rats (a, b; e, f) and hypothermic (synthetic torpor, ST) rats (c, d; g, h). Open arrows indicate MP neurons that showed moderate-to-bright AT8 immunoreactivity in the ileum of CTRL rats (b) and faint AT8 immunoreactivity in the colon (f). In the ST rats, MP neurons (open arrows) showed bright AT8 immunoreactivity in both the intestinal segments (d, h). Stars indicate some of the neuronal nuclei stained with DAPI (a, c, e, g), which were recognizable for their shape, dimension and staining intensity. White arrows indicate the nuclei of some glial cells, which were smaller than the neuronal nuclei. Elongated open arrows indicate the nuclei of some smooth muscle cells. Bar: a–h = 50 µm