Fig. 4.

Photomicrographs of whole-mount preparations of myenteric plexus (MP) of the ileum (a–f) and the colon (g–l) of normothermic (CTRL) (a–c; g–i) and hypothermic (synthetic torpor, ST) rats (d–f; j–l) showing AT8 and choline acetyltransferase (ChAT) immunolabeling. Open arrows indicate MP neurons co-expressing AT8 (a, d, g, j) and ChAT (b, e, h, k) immunoreactivity. White stars indicate cholinergic neurons, which were AT8 negative. Open stars (g–l) indicate AT8 immunoreactive neurons, which were ChAT negative. In (c, f, i) and (l), the merge images. Bar: (a–l)= 50 µm