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. 2021 May 15;29:19–29. doi: 10.1016/j.jot.2021.04.003

Figure 5.

Figure 5

KLF10 regulated IL-1β induced human NP cells degenerative phenotype. (A–D) The mRNA levels of MMP-3, MMP-13, aggrecan and collagen II in NP cells of each group as treated above was detected by qRT-PCR assay. (E, F) The representative MMP3 and collagen II were detected by the immunofluorescence. Scale bars: 50 ​μm. (G, H) The protein levels of KLF10, MMP3, MMP13, ADAMTS4, ADAMTS5, aggrecan and collagen II were detected by western blot. All experiments were repeated three times, and GAPDH is used as an internal control. Data are presented as the mean ​± ​SD. ∗, P<0.05; ∗∗, P<0.01; ∗∗∗, P<0.001.