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. 2021 May 15;29:19–29. doi: 10.1016/j.jot.2021.04.003

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

KLF10 regulate IVDD through modulate TGF-β signaling pathway. (A, B) The protein levels of TGF-β1, P-smad2/3, Smad2/3 and Smad7 in NP cells of each group as treated above were determined by western blotting. (C) The NP cells apoptosis rate of each group was detected by flow cytometry. (D) The NP cells migratory ability of each group was determined by transwell assay. Scale bar: 200 μm. (E) The NP cells proliferation of each group was determined by CCK8 assay. All experiments were repeated three times, and GAPDH is used as an internal control. Data are presented as the mean ± SD. ∗, P<0.05; ∗∗, P<0.01; ∗∗∗, P<0.001.