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. 2021 May 10;12:628045. doi: 10.3389/fneur.2021.628045

Figure 11.

Figure 11

Primers amplify Borrelia 16S−23S internal transcribed spacer (ITS) region. Bobu primers generate an amplicon size of 600 bp. To increase the specificity Bobu internal primers were designed to generate an amplicon size of 195 bp. (A) External PCR: L-molecular weight marker of 100 bp, N-negative control, P-positive control, NTC-No Template Control, 1–12,577 amygdala, 2–12,577 pons, 3–12,577 spinal cord. (B) Internal PCR: L-molecular weight marker of 50 bp, N-negative control, P-positive control, NTC-No Template Control, 1–12,577 amygdala, 2–12,577 pons, 3–12,577 spinal cord. *Samples that were positive to Borrelial DNA.