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. 2021 May 10;11:654300. doi: 10.3389/fonc.2021.654300

Figure 4.

Figure 4

pH- and concentration-dependent emission of NaFl. (A) Normalized absorbance (in 0.9% NaCl solution; dashed line) and pH dependent emission spectra of NaFl solutions in different phosphate buffers (phosphate buffer pH 5.54, pH 7.35, and pH 8.04) obtained with a confocal laser scanning microscope (CLSM). (B) Concentration series of Na-Fl solutions (in 0.9% NaCl), and (C) the corresponding absorbance (in 0.9% NaCl solution; dashed line), and concentration-dependent CLSM emission spectra (solid lines). (D) Emission maxima λmax [nm] plotted against NaFl concentration [%] show a concentration-dependent red shift by about 5 nm.