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. 2021 May 10;11:595285. doi: 10.3389/fonc.2021.595285

Figure 6.

Figure 6

The combination of the two drugs can inhibit the growth of tumor in nude mice. (A) There were seven nude mice in the placebo group and six in other groups. The drug was administered, before half an hour, regularly every day. Simvastatin was 0.4 mg and Abiraterone acetate was 3 mg contained in every 100 μl suspension aid. The dosage volume was converted according to the body weight of the nude mice. There was significant difference between the two groups. Data are presented as mean ± Standard Error (SEM). (B) RT-PCR analysis of mRNA levels of SCAP, SREBF1, SREBF2, HMGCR, CYP17A1, relative mRNA levels were calibrated with β-actin. GraphPad one-way analysis of variance (ANOVA) Analysis the expression differences of related transcription genes. Data are presented as mean ± Standard Error of Mean (SEM). (C) Pla, placebo; Com, combination simvastatin with abiraterone acetate. Western blots showing relative protein expression in three independent experiments. (D) Immunohistochemistry (IHC) analysis of relative protein expression. Bar, 125 μm. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.