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. 2021 May 10;12:660002. doi: 10.3389/fmicb.2021.660002

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Copyright © 2021 Conde, Price-Carter, Cochard, Branger, Stevenson, Whittington, Bannantine and Biet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bioinformatic analysis pipeline. Details of steps performed by the IS900 RFLP in silico pipeline. From complete genome sequences, BstEII restriction site and IS900 sequence positions were identified. Both data sets are merged together to extract the BstEII fragment sizes from the genome sequence. Previous positions IS900 and sequence orientation are stored in a.rflp file for further analysis. BstEII fragment sizes are converted into migration distance based on calculation from an in vitro gel migration (see section “Materials and Methods”) and saved in the .tsv file for further visualization of the RFLP profile.