Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 10;12:660002. doi: 10.3389/fmicb.2021.660002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Conde, Price-Carter, Cochard, Branger, Stevenson, Whittington, Bannantine and Biet.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

IS900 restriction fragment length polymorphism (RFLP) “in vitro” versus “in silico” (A) An in silico RFLP analysis was developed using complete genomes. This automated procedure identified the BstEII restriction sites to obtain the exact size of all DNA fragments carrying a copy of the IS900 sequence. IS900 RFLP profiles were compared using fragment sizes (A) or (B) by digital visualization of the restriction fragments separated according to their size by mimicking their migration in agarose gel. (C) The IS900 RFLP profiles obtained by classical Southern blot method and hybridization to IS900 were used to find the approximate fragment sizes by band analysis software (D).