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. 2021 Apr 16;24:905–922. doi: 10.1016/j.omtn.2021.04.011

Figure 4.

Figure 4

miR-337-3p and miR-137 were targets of MIR210HG

(A) miR-337-3p and miR-137 were downregulated in endometrial carcinoma tissues (n = 40) compared with normal tissues (n = 20). (B) Negative correlation was found between MIR210HG and miR-337-3p and miR-137 expression in endometrial carcinoma patients (Pearson’s correlation coefficient analysis). (C) Pearson’s correlation coefficient analysis was used to analyze the relationship between HMGA2 and miR-337-3p/137 expression. (D) Sequence alignment of lncRNA MIR210HG 3-UTR with WT versus mutant potential miR-337-3p and miR-137 targeting sites. (E) Schematic diagram of the Ago2-RIP process. The association between MIR210HG, miR-337-3p/137, and Ago2 was ascertained by analyzing Ishikawa cell lysates. The level of lncRNA MIR210HG and miR-337-3p/137 analyzed using real-time PCR from Ago2 precipitate. ∗p < 0.05 versus IgG control. (F and G) MIR210HG or miR-337-3p/137 expression analyzed using quantitative real-time PCR in Ishikawa and HEC-1A cell lines. Data are presented as the mean ± SEM (n = 3 per group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.