Figure 6.

HMGA2 is a functional target of the MIR210HG-miR-337-3p/137 axis in regulating EMT

(A) Western blot analysis of the expression of EMT, TGF-β, and Wnt-pathway-related proteins in response to MIR210HG in Ishikawa and HEC-1A cell lines. (B) HMGA2, β-catenin, and E-cadherin analyzed using immunofluorescence staining in Ishikawa and HEC-1A. (C) Western blot analysis of the expression of EMT, TGF-β, and Wnt-related pathway proteins in response to HMGA2 in Ishikawa and HEC-1A cell lines. (D) immunofluorescence experiment. (E) Western blot analysis of the expression of EMT, TGF-β, and Wnt- pathway-related proteins in response to miR-337-3p/137 in Ishikawa and HEC-1A cell lines. (F) immunofluorescence experimental results. (G) Protein level of HMGA2 determined using western blot analysis after co-transfection with sh-MIR210HG and miR-337-3p/137 inhibitor. (H) Immunofluorescence experiment. Data are presented as the mean ± SEM, (n = 3 per group). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.