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. 2021 May 10;12:659955. doi: 10.3389/fphar.2021.659955

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Copyright © 2021 Meng, Zhang, AI, Wu and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of β-asarone on apoptosis in PC12 cells. (A) Apoptosis was measured by flow cytometry, and the percentage of early and late apoptotic cells was quantified. The left lower quadrant represents the normal cells, the right lower quadrant represents the early apoptosis cells, the right upper quadrant represents the late apoptosis cells, and the left upper quadrant represents the death cells. a, Control group; b, Aβ_1–42-alone treatment group; c, Aβ_1–42+β-asarone (10 μM) group; d, Aβ_1–42+β-asarone (30 μM) group; e, Aβ_1–42+β-asarone (60 μM) group. (B) Expression levels and quantification of Bcl-2/Bax and cleaved caspase-3 were examined by Western blot. ^△ p < 0.05 compared with the control group; ^* p < 0.05 compared with the Aβ_1–42-alone treatment group. The data were expressed as mean ± SD of three independent experiments, each in triplicate.