Figure 4.

Erastin (Era) and Propranolol (Prop) do not affect the differentiation route of monocytes into ECs. (A) Neither Era and/or Prop affect the expression of vWF, even when co-exposed with a short H₂O₂ stimulation (positive control of the differentiation pattern of monocytes), indicating that Era and/or Prop have no impact in the differentiation process of monocytes-derived cells into ECs. Era and/or Prop exposure before the short H₂O₂ stimulation does not influence the intracellular ROS (DCF-DA; B) and lipid peroxide (11C-Bodipy; C) levels. In (B, C) data are normalized to the control condition and represented as mean ± SD. *p<0.5, **p<0.01, ***p<0.001, ****p<0.0001.