Figure 2.

Hepatocyte miR-34a promoted lipid absorption and synthesis but inhibited FAO. The mice are described in the legend in Figure 1 (n = 8 per group). (A) mRNA levels of genes involved in cholesterol metabolism. (B) mRNA levels of genes involved in fatty acid metabolism. (C and D) Western blotting assays (C) were performed and protein levels were quantified (D). (E) Bile acid (BA) pool size (left panel) and plasma BA levels (right panel). (F) Intestinal fat absorption. (G) Intestinal cholesterol absorption. (H) Newly synthesized TGs were quantified by GC–MS. (I) Mouse primary hepatocytes were isolated from mice infected with AAV8-ALB-Null or AAV8-ALB-miR-34a and FAO was determined using ³H-palmitic acid as substrates (n = 5). ∗P < 0.05 and ∗∗P < 0.01.