Figure 4.
Loss of hepatocyte miR-34a attenuated diet-induced steatohepatitis. The miR-34afl/fl mice and miR-34aHep−/− mice were fed an HFCF diet for 16 weeks (n = 7–8 per group). (A) Plasma ALT and AST levels. (B) Hepatic TC, FC, and CE levels. (C) Hepatic TG levels. (D) Hepatic FFA levels. (E) Hepatic individual fatty acyl-CoA levels. (F) Hepatic hydroxyproline levels. (G) Liver sections were immunostained using an F4/80 antibody and the F4/80-positive area was determined. (H) Representative liver images of Oil Red O staining (upper panel) or Picrosirius red staining (bottom panel). ∗P < 0.05 and ∗∗P < 0.01.