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. 2021 Apr 28;51:101244. doi: 10.1016/j.molmet.2021.101244

Figure 5.

Figure 5

Loss of hepatocyte miR-34a inhibited lipid absorption and synthesis and induced FAO. (A-I) The mice are described in the legend in Figure 4 (n = 7–8 mice per group). Hepatic mRNA (A and B) and protein levels were determined (C and D). BA pool size (left panel) and plasma BA levels were analyzed (E). Intestinal fat absorption (F), intestinal cholesterol absorption (G), and hepatic newly synthesized TG levels (H) were measured. FAO was determined using hepatocytes isolated from the miR-34afl/fl mice or miR-34aHep−/− mice (I). (J-M) The miR-34afl/fl mice and miR-34aHep−/− mice were i.v. injected with 0.5 × 10ˆ11 GC AAV8-ALB-Null or AAV8-ALB-CYP7A1 plus AAV8-ALB-CYP8B1 (7A1 + 8B1). These mice were then fed an HFCF diet for 16 weeks (n = 8 per group). Hepatic TC (J), FC (K), TG (L), and FFA (M) levels were determined. ∗P < 0.05 and ∗∗P < 0.01.