Figure 6.

Loss of hepatocyte miR-34a inhibited Kupffer cell activation and apoptosis. The mice are described in the legend in Figure 4 (n = 7–8 mice per group). (A) Hepatic mRNA levels. (B and C) Hepatic protein levels. (D) Hepatic apoptotic cells (%) as determined by TUNEL assays. (E) Hepatic ROS levels. (F) Hepatic MDA levels. (G) Kupffer cells were incubated with exosomes isolated from the miR-34a^fl/fl mice or miR-34a^Hep−/− mice. miR-34a levels in exosomes and mRNA levels in Kupffer cells were determined (n = 4). ∗P < 0.05 and ∗∗P < 0.01.