Skip to main content
. 2021 May 10;12:671034. doi: 10.3389/fphys.2021.671034

Figure 5.

Figure 5

ARF6 acts a GTPase activating protein (GAP) substrate for ARFGAP1-mediated endocytic sorting. (A) Single point uptake of GFP-EAAT3-WT and GFP-EAAT3-F508A co-expressed with CFP-ARF6 WT or CFP-ARF6-Q67L in the ratio of 1:1. Data represents mean ± SD (n = 4); ***p = 0.0005, one way ANOVA followed by Sidak’s multiple comparisons test; p = 0.8517 between EAAT3 WT Control and WT:ARF6 WT(1:1); *p = 0.0256 between EAAT3 WT Control and WT:ARF6 Q67L(1:1); ***p = 0.0010 between EAAT3 WT Control and EAAT3 F508A; p = 0.8977 between EAAT3 F508A and WT:ARF6 Q67L(1:1); ns-not significant. (B–F) Confocal image of GFP-EAAT3-WT or GFP-EAAT3-F508A cotransfected with CFP-ARF6-WT or CFP-ARF6-Q67L demonstrating the loss of endocytosis in the presence of CFP-ARF6-Q67L with no effect on GFP-EAAT3 F508A. Scale bar: 5 μm.