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. 2021 May 10;9:607057. doi: 10.3389/fcell.2021.607057

FIGURE 2.

FIGURE 2

Expression domains of amphioxus CollagenA (ColA) and CollagenIV (Col4) genes. (A,B) Expression pattern of ColA (A,A′) N4 stage embryo, lateral and dorsal views. (B) Cross section of N4 embryo in panel (A′) at the level marked with a white dashed line. (C–I) Expression pattern of Col4a1/3/5. (C,C′) N0 stage embryo, lateral and dorsal view. (D,D′) N3 stage embryo, lateral and dorsal view. (E,E′) N5 stage embryo, lateral and dorsal view. (F) T1 stage larva, lateral view. (G) L2 stage larva, lateral view. (H) Cross section of the N5 embryo in panel (E) at the level marked with a white dashed line. (I) Cross section of the T1 larva in panel (F) at the level marked with a white dashed line. (J–P) Expression pattern of Col4a2/4/6. (J,J′) N0 stage embryo, lateral and dorsal view. (K,K′) N3 stage embryo, lateral and dorsal view; Col4a2/4/6 is expressed in the lateral part of the somite and minimally in the endoderm. (L,L′) N5 stage embryo, lateral and dorsal view. (M) T1 stage larva, lateral view. (N) L2 stage larva, lateral view. (O) Cross section of the N5 stage embryo in panel (L) at the level marked with a white dashed line. (P) Cross section of T1 stage larva in panel (M) at the level marked with a white dashed line. (Q) Cross section of N4 stage double FISH of Col4a1/3/5 with mActin.