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. 2021 May 10;9:660576. doi: 10.3389/fcell.2021.660576

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Copyright © 2021 Fa, Zhang, Zhang, Qi, Zhang, Fu, Xu, Gao and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of lnc-TSSK2-8 activates the Wnt/β-catenin signaling pathway. (A) RT-qPCR quantification of overexpression lnc-TSSK2-8 in HEK293 cells. (B) Flow cytometry analysis of cell proliferation. The transcriptional level of lymphoid enhancer-binding factor 1 (LEF1) (C) and β-catenin (CTNNB1) (D) in HEK293 cells with lnc-TSSK2-8 overexpression. (E) The influence of lnc-TSSK2-8 overexpression on protein expression and phosphorylation of β-catenin. (F) The influence of lnc-TSSK2-8 overexpression on the activity of glycogen synthase kinase 3β (GSK-3β). (G) The influence of lnc-TSSK2-8 overexpression on ubiquitination of β-catenin. *p < 0.05, **p < 0.001, **p < 0.0001.