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. 2021 May 10;9:660576. doi: 10.3389/fcell.2021.660576

FIGURE 4.

FIGURE 4

lnc-TSSK2-8 activation promotes β-catenin stabilization through miR-6721-5p/heat shock protein (HSP)A6 axis. (A) The predicted miR-6721-5p binding sites of lnc-TSSK2-8 and HSPA6 3′ UTR. (B) The luciferase reporter assay we performed to confirm the interaction between miR-6721-5p and HSPA6 3′ UTR (B)/lnc-TSSK2-8 (C). (D) RT-qPCR analysis of lnc-TSSK2-8 level in response to miR-6721-5p overexpression. (E) RT-qPCR analysis of β-catenin level in response to miR-6721-5p overexpression. (F) Western blot (WB) analysis of protein expression and phosphorylation of β-catenin in response to miR-6721-5p overexpression. (G) RT-qPCR analysis of HSPA6 level in response to lnc-TSSK2-8 overexpression. (H) Confirmation of HSPA6 overexpression with RT-qPCR. (I) Confirmation of HSPA6 overexpression with WB. (J) RT-qPCR analysis of LEF1 level in response to HSPA6 overexpression. (K) RT-qPCR analysis of β-catenin level in response to HSPA6 overexpression. (L) WB analysis of β-catenin protein expression and phosphorylation in response to HSPA6 overexpression. *p < 0.05, **p < 0.001, **p < 0.0001.