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. 2021 May 10;9:660576. doi: 10.3389/fcell.2021.660576

FIGURE 5.

FIGURE 5

lnc-TSSK2-8 activation promotes β-catenin stabilization through the miR-491-5p/α-B-crystallin (CRYAB) axis. (A) The predicted miR-491-5p binding sites of lnc-TSSK2-8 and CRYAB 3′ UTR. (B) The luciferase reporter assay we performed to confirm the interaction between miR-6721-5p and CRYAB 3′ UTR (B)/lnc-TSSK2-8 (C). (D) RT-qPCR analysis of CRYAB level in response to miR-491-5p overexpression. (E) RT-qPCR analysis of lymphoid enhancer-binding factor 1 (LEF1) level in response to miR-491-5p overexpression. (F) RT-qPCR analysis of β-catenin level in response to miR-491-5p overexpression. (G) Western blot (WB) analysis of protein expression of β-catenin in response to miR-491-5p overexpression. (H) Confirmation of lnc-TSSK2-8 overexpression with RT-qPCR. (I) RT-qPCR analysis of CRYAB level in response to lnc-TSSK2-8 overexpression. (J) Confirmation of CRYAB overexpression with RT-qPCR. (K) Confirmation of CRYAB overexpression with WB. (L) RT-qPCR analysis of β-catenin level in response to CRYAB overexpression. (M) WB analysis of β-catenin protein expression and phosphorylation in response to CRYAB overexpression. *p < 0.05, **p < 0.001, **p < 0.0001.